基本信息：

中文名称：

ActivAbTMCELF1 抗体封闭肽

英文名称：

ActivAbTMCELF1 Antibody Blocking Peptide

别名：

50 kDa Nuclear polyadenylated RNA binding protein,50 kDa nuclear polyadenylated RNA-binding protein,Bruno like 2,bruno like protein 2,Bruno-like protein 2,BRUNOL 2,BRUNOL2,CELF 1,CELF-1,celf1,CELF1 CUGBP,Elav like family member 1,CELF1_HUMAN,CUG BP and ETR 3 like factor 1,CUG BP,CUG BP1,CUG RNA binding protein,CUG triplet repeat RNA binding protein 1,CUG triplet repeat RNA-binding protein 1,CUG-BP,CUG-BP- and ETR-3-like factor 1,CUG-BP1,CUGBP 1,CUGBP and ETR3 like factor 1,CUGBP,CUGBP Elav like family member 1,CUGBP Elav-like family member 1,CUGBP1,Cytidine uridine guanosine binding protein 1,Deadenylation factor CUG BP,Deadenylation factor CUG-BP,Deadenylation factor CUGBP,EDEN BP,EDEN BP homolog,EDEN-BP,EDEN-BP homolog,embryo deadenylation element binding protein,embryo deadenylation element binding protein homolog,Embryo deadenylation element-binding protein homolog,hNab 50,hNab50,NAB 50,NAB50,NAPOR,Nuclear polyadenylated RNA binding protein 50 kD,Nuclear polyadenylated RNA binding protein,RNA binding protein BRUNOL 2,RNA binding protein BRUNOL2,RNA-binding protein BRUNOL-2

外观(性状)：

Lyophilized powder

来源：

Synthetic

储存条件：

Store at -20°C. Avoid freeze / thaw cycles.

运输条件：

冷冻运输

规格：

500ug



生物活性：

应用：

Blocking Peptide of K108975P Antibody

纯化方法：

HPLC

背景说明：

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA.

生物活性：

Not tested

该产品为 CELF1 抗体的抗原肽，在抗体检测、纯化时作为封闭肽使用，对应抗体货号为：K108975P。
抗体封闭肽是一类专门用于免疫学检测体系的功能性合成多肽，其核心作用是通过特异性结合干扰性抗体或非特异性结合位点，从而降低实验背景信号，提高检测结果的准确性和可靠性。它并不直接识别目标抗原，而是充当一种“分子屏蔽层”，封闭那些可能导致假阳性或假阴性结果的非特异性相互作用。
在功能层面，抗体封闭肽主要具备两大特性。一是高亲和力的特异性中和能力——其氨基酸序列与检测体系中可能引起交叉反应的抗体表位完全相同或高度同源，因此能够优先与这些干扰抗体结合，占据其活性位点。二是物理屏蔽作用，即在不干扰靶标抗原与检测抗体正常识别的前提下，封闭固相载体或捕获分子上的空缺结合区域，减少杂蛋白或杂抗体的非特异性吸附。
在应用层面，抗体封闭肽广泛应用于各类免疫分析技术中，包括酶联免疫吸附测定、蛋白质印迹、免疫组化以及免疫层析试纸条等。在自身抗体检测、生物制剂的免疫原性分析以及多重免疫检测方法开发中，它能够有效消除由异嗜性抗体、抗物种抗体或样本基质效应引起的背景干扰。此外，在基于标签融合蛋白的表达检测以及抗体亲和纯化流程中，封闭肽同样扮演着关键角色，可作为竞争性洗脱剂或信号校正控制组使用。
注意事项：
1.本产品仅供科研使用，请勿用于医药、临床治疗、食品及化收品等用途 。
2.为了您的安全和健康，请穿实验服，口罩，护目镜，并戴一次性手套操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
4.请勿存放于普通住宅区。

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