基本信息：

中文名称：

ActivAbTMCDK2 抗体封闭肽

英文名称：

ActivAbTMCDK2 Antibody Blocking Peptide

别名：

Cdc2 related protein kinase,cdc2-related protein kinase,Cdk 2,CDK2_HUMAN,Cell devision kinase 2,Cell division kinase 2,Cell division protein kinase 2,Cyclin dependent kinase 2,cyclin dependent kinase 2-alpha,Cyclin-dependent kinase 2,p33 protein kinase,p33(CDK2)

外观(性状)：

Lyophilized powder

来源：

Synthetic

储存条件：

Store at -20°C. Avoid freeze / thaw cycles.

运输条件：

冷冻运输

规格：

500ug



生物活性：

应用：

Blocking Peptide of K108755P Antibody

纯化方法：

HPLC

背景说明：

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability. Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.

生物活性：

Not tested

该产品为 CDK2 抗体的抗原肽，在抗体检测、纯化时作为封闭肽使用，对应抗体货号为：K108755P。
抗体封闭肽是一类专门用于免疫学检测体系的功能性合成多肽，其核心作用是通过特异性结合干扰性抗体或非特异性结合位点，从而降低实验背景信号，提高检测结果的准确性和可靠性。它并不直接识别目标抗原，而是充当一种“分子屏蔽层”，封闭那些可能导致假阳性或假阴性结果的非特异性相互作用。
在功能层面，抗体封闭肽主要具备两大特性。一是高亲和力的特异性中和能力——其氨基酸序列与检测体系中可能引起交叉反应的抗体表位完全相同或高度同源，因此能够优先与这些干扰抗体结合，占据其活性位点。二是物理屏蔽作用，即在不干扰靶标抗原与检测抗体正常识别的前提下，封闭固相载体或捕获分子上的空缺结合区域，减少杂蛋白或杂抗体的非特异性吸附。
在应用层面，抗体封闭肽广泛应用于各类免疫分析技术中，包括酶联免疫吸附测定、蛋白质印迹、免疫组化以及免疫层析试纸条等。在自身抗体检测、生物制剂的免疫原性分析以及多重免疫检测方法开发中，它能够有效消除由异嗜性抗体、抗物种抗体或样本基质效应引起的背景干扰。此外，在基于标签融合蛋白的表达检测以及抗体亲和纯化流程中，封闭肽同样扮演着关键角色，可作为竞争性洗脱剂或信号校正控制组使用。
注意事项：
1.本产品仅供科研使用，请勿用于医药、临床治疗、食品及化收品等用途 。
2.为了您的安全和健康，请穿实验服，口罩，护目镜，并戴一次性手套操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
4.请勿存放于普通住宅区。

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