基本信息：

中文名称：

ActivAbTMZFP36L1 抗体封闭肽

英文名称：

ActivAbTMZFP36L1 Antibody Blocking Peptide

别名：

Berg36,BRF1,Butyrate response factor 1,C3H1 type-like 1,cMG1,EGF-response factor 1,ERF-1,ERF1,Protein TIS11B,RNF162B,TIS11B,TISB_HUMAN,ZFP36L1,Zinc finger protein 36

外观(性状)：

Lyophilized powder

来源：

Synthetic

储存条件：

Store at -20°C. Avoid freeze / thaw cycles.

运输条件：

冷冻运输

规格：

500ug



生物活性：

应用：

Blocking Peptide of K110803P Antibody

纯化方法：

HPLC

背景说明：

Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:12198173, PubMed:15538381, PubMed:15467755, PubMed:17030608, PubMed:19179481, PubMed:20702587, PubMed:24700863, PubMed:25106868, PubMed:25014217, PubMed:26542173). Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (PubMed:15687258). Functions by recruiting the CCR4-NOT deadenylase complex and components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes (PubMed:15687258, PubMed:18326031, PubMed:25106868). Induces also the degradation of ARE-containing mRNAs even in absence of poly(A) tail (By similarity). Binds to 3'-UTR ARE of numerous mRNAs (PubMed:12198173, PubMed:15538381, PubMed:15467755, PubMed:17030608, PubMed:19179481, PubMed:20702587, PubMed:24700863, PubMed:25106868, PubMed:25014217, PubMed:26542173). Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs) (By similarity). Promotes ARE-mediated mRNA decay of mineralocorticoid receptor NR3C2 mRNA in response to hypertonic stress (PubMed:24700863). Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA (PubMed:20702587). Positively regulates monocyte/macrophage cell differentiation by promoting ARE-mediated mRNA decay of the cyclin-dependent kinase CDK6 mRNA (PubMed:26542173). Promotes degradation of ARE-containing pluripotency-associated mRNAs in embryonic stem cells (ESCs), such as NANOG, through a fibroblast growth factor (FGF)-induced MAPK-dependent signaling pathway, and hence attenuates ESC self-renewal and positively regulates mesendoderm differentiation (By similarity). May play a role in mediating pro-apoptotic effects in malignant B-cells by promoting ARE-mediated mRNA decay of BCL2 mRNA (PubMed:25014217). In association with ZFP36L2 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity-joining (VDJ) recombination and functional immune cell formation (By similarity). Together with ZFP36L2 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA (By similarity). Participates in the delivery of target ARE-mRNAs to processing bodies (PBs) (PubMed:17369404). In addition to its cytosolic mRNA-decay function, plays a role in the regulation of nuclear mRNA 3'-end processing; modulates mRNA 3'-end maturation efficiency of the DLL4 mRNA through binding with an ARE embedded in a weak noncanonical polyadenylation (poly(A)) signal in endothelial cells (PubMed:21832157). Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion (PubMed:15967811). Plays a role in vasculogenesis and endocardial development (By similarity). Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis (PubMed:27182009). Plays a role in myoblast cell differentiation (By similarity).

生物活性：

Not tested

该产品为 ZFP36L1 抗体的抗原肽，在抗体检测、纯化时作为封闭肽使用，对应抗体货号为：K110803P。
抗体封闭肽是一类专门用于免疫学检测体系的功能性合成多肽，其核心作用是通过特异性结合干扰性抗体或非特异性结合位点，从而降低实验背景信号，提高检测结果的准确性和可靠性。它并不直接识别目标抗原，而是充当一种“分子屏蔽层”，封闭那些可能导致假阳性或假阴性结果的非特异性相互作用。
在功能层面，抗体封闭肽主要具备两大特性。一是高亲和力的特异性中和能力——其氨基酸序列与检测体系中可能引起交叉反应的抗体表位完全相同或高度同源，因此能够优先与这些干扰抗体结合，占据其活性位点。二是物理屏蔽作用，即在不干扰靶标抗原与检测抗体正常识别的前提下，封闭固相载体或捕获分子上的空缺结合区域，减少杂蛋白或杂抗体的非特异性吸附。
在应用层面，抗体封闭肽广泛应用于各类免疫分析技术中，包括酶联免疫吸附测定、蛋白质印迹、免疫组化以及免疫层析试纸条等。在自身抗体检测、生物制剂的免疫原性分析以及多重免疫检测方法开发中，它能够有效消除由异嗜性抗体、抗物种抗体或样本基质效应引起的背景干扰。此外，在基于标签融合蛋白的表达检测以及抗体亲和纯化流程中，封闭肽同样扮演着关键角色，可作为竞争性洗脱剂或信号校正控制组使用。
注意事项：
1.本产品仅供科研使用，请勿用于医药、临床治疗、食品及化收品等用途 。
2.为了您的安全和健康，请穿实验服，口罩，护目镜，并戴一次性手套操作。
3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
4.请勿存放于普通住宅区。

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